Solubilization of human erythrocyte membranes by ASB detergents
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چکیده
منابع مشابه
Solubilization of human erythrocyte membranes by ASB detergents.
Understanding the membrane solubilization process and finding effective solubilizing agents are crucial challenges in biochemical research. Here we report results on the interaction of the novel linear alkylamido propyl dimethyl amino propanosulfonate detergents, ASB-14 and ASB-16, with human erythrocyte membranes. An estimation of the critical micelle concentration of these zwitterionic deterg...
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enzymes is due to a more specific process than generalizedcell damage or loss (Holdsworth & Coleman, 1975). We have now attempted to make an initial separation of these enzymes from other biliary proteins, before investigating their relationship with the bile-canaliculus region of the hepatocyte plasma membrane. Fresh sheep bile was treated with solid protamine sulphate and centrifuged to remov...
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The kinetics of [3H]bilirubin binding to human erythrocyte ghost membranes was investigated. The binding occurred rapidly and was saturable with respect to [3H]bilirubin and membrane concentration. The apparent dissociation constant (Kd) and maximum binding (Bmax.) for bilirubin of the membranes were 2.3 microM and 0.93 nmol/mg of protein respectively. Low-affinity binding, non-saturable at 400...
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The effects of pH, trypsin, and phospholipase C on the topographic distribution of acidic anionic residues on human erythrocytes was investigated using colloidal iron hydroxide labeling of mounted, fixed ghost membranes. After glutaraldehyde fixation at pH 6.5-7.5, the positively charged colloidal particles were bound to the membranes in small randomly distributed clusters. The clusters of anio...
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ژورنال
عنوان ژورنال: Brazilian Journal of Medical and Biological Research
سال: 2008
ISSN: 0100-879X
DOI: 10.1590/s0100-879x2008000900003